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Metabolic pathways

Every FMK pathway as a clickable map. Pick one pathway to hammer it — next step, enzyme, regulator, cofactor, and the facts that get tested (NADPH and who needs it, rate-limiting steps, deficiencies) — or set it to all pathways for a mixed drill. Every answer says which enzyme and which pathway.

Pathway
Ethanol metabolism · Hepatocyte cytosol (ADH), mitochondria (ALDH2), smooth ER (MEOS/CYP2E1), peroxisome (catalase). Enzymes sit beside the arrows; a ★ marks the rate-limiting step. The map scrolls inside its panel.
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EthanolEthanolAcetaldehydeAcetaldehydeAcetateAcetateAcetyl-CoAAcetyl-CoA↑ NADH / NAD⁺ ratio↑ NADH / NAD⁺ ratioPyruvate → lactatePyruvate → lactateOAA → malateOAA → malateDHAP → glycerol-3-P → TGDHAP → glycerol-3-P → TG↓ β-oxidation↓ β-oxidation↑ Ketogenesis↑ KetogenesisAlcohol dehydrogenase (ADH)Aldehyde dehydrogenase (ALDH2,mitochondrial)Acetyl-CoA synthetase (peripheraltissues)Net: 2 NADH per ethanolLactate dehydrogenase drivenforwardMalate dehydrogenase drivenbackwardsGlycerol-3-P dehydrogenase drivenforward3-Hydroxyacyl-CoA dehydrogenaseinhibitedHMG-CoA synthase / lyase(mitochondria)

Ethanol metabolism

Ethanol → acetaldehyde (ADH, NAD⁺ → NADH) → acetate (ALDH, NAD⁺ → NADH) → acetyl-CoA. Two NADH per ethanol flood the liver: pyruvate → lactate, OAA → malate, DHAP → glycerol-3-P, β-oxidation inhibited. Result: hypoglycemia (fasting), lactic acidosis, steatosis, ketoacidosis. Chronic use induces MEOS (CYP2E1, NADPH, ROS).

Click any metabolite on the map for its reactions. Source: FMK-03.4 Fructose, galactose & ethanol.

Reactions

StepEnzymeCofactorsRegulation
Ethanol → Acetaldehyde Alcohol dehydrogenase (ADH)Methanol poisoning →Ethylene glycol (antifreeze) poisoning → NAD⁺ → NADH
Ethanol → Acetaldehyde MEOS — CYP2E1 (smooth ER)Acetaminophen hepatotoxicity in chronic alcohol use (CYP2E1/NAPQI) → NADPH + O₂
Acetaldehyde → Acetate Aldehyde dehydrogenase (ALDH2, mitochondrial)
ALDH2 polymorphism / disulfiram — acetaldehyde accumulation: flushing, headache, nausea, hypotension after drinking
ALDH2 deficiency ('Asian flush', rs671) →Disulfiram (Antabuse) - acetaldehyde reaction →
NAD⁺ → NADH
Disulfiram; Metronidazole
Acetate → Acetyl-CoA Acetyl-CoA synthetase (peripheral tissues) ATP
Acetaldehyde → ↑ NADH / NAD⁺ ratio Net: 2 NADH per ethanol
↑ NADH / NAD⁺ ratio → Pyruvate → lactate Lactate dehydrogenase driven forward
↑ NADH / NAD⁺ ratio → OAA → malate Malate dehydrogenase driven backwards
↑ NADH / NAD⁺ ratio → DHAP → glycerol-3-P → TG Glycerol-3-P dehydrogenase driven forward
↑ NADH / NAD⁺ ratio → ↓ β-oxidation 3-Hydroxyacyl-CoA dehydrogenase inhibited
Acetyl-CoA → ↑ Ketogenesis HMG-CoA synthase / lyase (mitochondria)

Conditions that live on this map

Acetaminophen hepatotoxicity in chronic alcohol use (CYP2E1/NAPQI)Alcohol-induced (alcoholic) hypoglycemiaAlcoholic liver disease (steatosis → hepatitis → fibrosis → cirrhosis)ALDH2 deficiency ('Asian flush', rs671)Disulfiram (Antabuse) - acetaldehyde reactionEthylene glycol (antifreeze) poisoningMethanol poisoning

Blue pills sit on one specific arrow; grey ones are whole-pathway problems. Each opens the full condition card, which links back here with the arrow lit.

Facts worth knowing

Enzyme deficiency

  • Alcoholic hepatitis lab pattern — AST > ALT (≥2:1; alcohol depletes PLP, ALT needs more), ↑GGT, ↑MCV, ↑bilirubin, ↑INR
  • Alcoholic ketoacidosis vs DKA — the glucose clue — AKA: glucose normal or low, β-OHB ≫ acetoacetate; DKA: glucose high. AKA needs fluids + glucose + thiamine, no insulin
  • Drug that irreversibly inhibits ALDH for aversive therapy — Disulfiram — 'FIRES up acetaldehyde accumulation'
  • Deficiency of Aldehyde dehydrogenase (ALDH2, mitochondrial) causes… — ALDH2 polymorphism / disulfiram — acetaldehyde accumulation: flushing, headache, nausea, hypotension after drinking (ALDH2*2 (rs671, ~40% of East Asians) → acetaldehyde accumulates → flushing, nausea, tachycardia (same as disulfiram); ↑ esophageal cancer risk.)

Regulation

  • Order of treatment in a hypoglycemic chronic drinker — Thiamine FIRST, then dextrose — glucose without B₁ can precipitate Wernicke encephalopathy

Cofactor

  • Which pathway uses NADPH instead of NAD⁺ to oxidise ethanol? — MEOS (CYP2E1) — inducible, generates ROS
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